Power failure or a jammed mechanical emergency release.
: Ensure the rotor is properly seated on the drive shaft and the knurled nut is tightened securely. Lid Mechanism
Locate the small plastic plug or opening on the bottom or side panel of the unit.
Eppendorf 5402 is a classic refrigerated microcentrifuge designed for temperature-sensitive sample processing in laboratory environments. While it is a legacy model, its core operational and maintenance principles remain consistent with current Eppendorf safety standards. Core Specifications Speed Range: 14,000 RPM (approx. 16,000 x g) adjustable in 100 RPM increments. Temperature Control: Adjustable from -9°C to 40°C Digital control from 1 to 99 minutes plus a momentary "Short Spin" button for brief cycles. Typically accommodates up to 18 x 1.5/2.0 mL tubes depending on the rotor used. GMI - Trusted Laboratory Solutions Operating Instructions Lid Access: Ensure the centrifuge is plugged in and the power switch is to disengage the electronic lid lock. Rotor Loading: Always load the rotor symmetrically eppendorf 5402 manual updated
| Feature / Parameter | Original Manual (Pre-2005) | Updated Manual (2020+) | Action Required | | :--- | :--- | :--- | :--- | | | 14,000 rpm (F-45-30-11) | 13,200 rpm (derated due to fatigue) | Reduce max speed by 5% for old rotors | | Temperature Range | -9°C to +40°C | -5°C to +40°C (below -5°C only with pre-cooling) | Avoid running below -5°C for >2 hours | | Imbalance Cut-off | >50g difference | >30g difference (stricter tolerance) | Balance buckets within 0.5g | | Lid Lock Mechanism | Manual release via hex key | Emergency release via pull-cord (behind front panel) | Practice emergency release quarterly | | Aerosol Sealing | "Not certified" | "No aerosol-tight certification for 5402" (explicit warning) | Do not use for pathogenic samples without biosafety buckets |
Older centrifuge models like the 5402 often rely on a motor with brushes, a consumable part that wears down over time. Checking the brushes is a key maintenance task. Here’s the general procedure (always consult your manual):
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This guide serves as an updated overview of the operational manual, maintenance schedules, and troubleshooting techniques for the Eppendorf 5402 Go to product viewer dialog for this item. 1. Introduction to the Eppendorf 5402 Eppendorf 5402 Go to product viewer dialog for this item.
Wait for the digital display to reach the target temperature before loading samples. Step 2: Loading the Rotor
Below is a table of common issues, potential causes, and solutions for the Eppendorf 5402. Always refer to the official manual for detailed troubleshooting charts. 16,000 x g) adjustable in 100 RPM increments
Close the lid tightly until the lock clicks into place. Run Parameter Selection: Set the Speed (RPM/RCF) and Time.
The is a legacy refrigerated benchtop microcentrifuge known for its reliability in molecular biology applications. While newer models like the Centrifuge 5420 have succeeded it, maintaining the 5402 requires adhering to original technical specifications and updated maintenance standards. Core Technical Specifications Maximum Speed: 14,000 RPM (approx. 16,000 x g). Temperature Range: -4∘Cnegative 4 raised to the composed with power C 40∘C40 raised to the composed with power C , providing stable cooling for sensitive samples.
Operating at sub-ambient temperatures causes ice buildup on the chamber walls. Switch off the centrifuge and unplug the power cord. Leave the lid open to let the ice melt naturally. Soak up the melted water with a cloth. Wipe the chamber dry before restarting the unit. 5. Updated Troubleshooting and Error Codes